Main Article Content

Abstract

Nowadays, many laboratory and traditional methods have been developed to determine the quality of honey, which each of them has own characteristics. Usually, traditional methods are quick, easy and cheap, but they have less accuracy, unlike, laboratory methods have sufficient accuracy, but require more costs and facilities. Honey is an ancient valuable food and in most cases has enchanted its consumers by its medic characteristics. Unfortunately, in recent years honey frauds have increased, which has led to distrust and lack of interest of consumers in this valuable product. Recognition of adulterated and poor quality honey in most countries are done in two ways; Laboratory and traditional. Spectroscopy methods are among the most widely used, fast, accurate and non-destructive methods, in addition, chromatography methods are extremely accurate but require more time and money.

Keywords

Honey Traditional method Laboratory method Fraud Spectroscopy Chromatography

Article Details

How to Cite
Sarwary, N. A. (2025). Studying the application of precise laboratory and traditional methods to identify pure honey. Journal of Natural Sciences – Kabul University, 6(3), 175–187. https://doi.org/10.62810/jns.v6i3.341

References

  1. Ashtiyani,S. Shamsi, M. Hosseini, N. Ramezani,M,. Honey in Quran and medicine. Arak Medical University Journal. 2011. Pp 51-57.
  2. Suarez,J,M. Tulipani,S. Romandini,S. Bertoli,E. Battino,M. Contribution of honey in nutrition and human health. Journal of Springer. 2010. Pp 3:15-23.
  3. Fakhlaei,R. Selmat,J. Khatib,A. Razis,A,F. Sukor,R et al. The Toxic Impact of Honey Adulteration. Journal of Foods. 2020. Pp 1-21.
  4. Johnson, R.J.; Fuggle, S.V.; Mumford, L.; Bradley, J.A.; Forsythe, J.L.; Rudge, C.J.; Kidney Advisory Group of NHS Blood and Transplant. A New UK 2006 National Kidney Allocation Scheme for deceased heart-beating donor kidneys Transplantation. 2010, 89, 387–394.
  5. Soares, S.; Amaral, J.S.; Oliveira, M.B.P.; Mafra, I. A comprehensive review on the main honey authentication issues: Production and origin. Compr. Rev. Food Sci. Food Saf. 2017, 16, 1072–1100.
  6. Food and Agriculture Organization of the United Nations (FAO); World Health Organization (WHO). Joint FAO/WHO Food Standard Programme Codex Alimentarius Commission Twenty-Fourth Session Geneva, 2–7 July 2001. In Codex Alimentarius Commission; ALINORM 01/34A; FAO: Rome, Italy; WHO: Geneva, Switzerland. 2001.
  7. Samat, S.; Kanyan Enchang, F.; Nor Hussein, F.; Wan Ismail, W.I. Four-week consumption of Malaysian honey reduces excess weight gain and improves obesity-related parameters in high fat diet induced obese rats. Evid. Based Complement. Altern. Med. 2017.
  8. Azar,M. Yosofpoor,M. Azar,S. Aghajani,S. Point of view of Islam and traditional medicine and modern about Honey. Journal of Clinical Excellence. 2015. Pp 4(1); 44-56.
  9. Zábrodská B, Vorlová L. Adulteration of honey and available methods for detection–a review. Acta Veterinaria Brno. 2015 May 5;83(10):85-102.
  10. Guler A, Bakan A, Nisbet C, Yavuz O. Determination of important biochemical properties of honey to discriminate pure and adulterated honey with sucrose (Saccharum officinarum L.) syrup. Food chemistry. 2007 Jan 1;105(3): 19-25.
  11. Thrasyvoulou A, Tananaki C, Goras G, Karazafiris E, Dimou M, Liolios V, Kanelis D, Gounari S. Legislation of honey criteria and standards. Journal of Apicultural Research. 2018 Jan 1;57(1):88-96.
  12. Hüfner S. Photoelectron spectroscopy: principles and applications. Springer Science & Business Media; 2013 Mar 9, 2-4.
  13. Naila A, Flint SH, Sulaiman AZ, Ajit A, Weeds Z. Classical and novel approaches to the analysis of honey and detection of adulterants. Food Control. 2018 Aug 1; 90: 52-65.
  14. Guelpa A, Marini F, du Plessis A, Slabbert R, Manley M. Verification of authenticity and fraud detection in South African honey using NIR spectroscopy. Food Control. 2017 Mar 1; 73: 88-96.
  15. Ruoff K, Karoui R, Dufour E, Luginbühl W, Bosset JO, Bogdanov S, Amadò R. Authentication of the botanical origin of honey by front-face fluorescence spectroscopy. A preliminary study. Journal of agricultural and food chemistry. 2005 Mar 9; 53(5): 3-7.
  16. Riswahyuli Y, Rohman A, Setyabudi FM, Raharjo S. Indonesian wild honey authenticity analysis using attenuated total reflectance-fourier transform infrared (ATR-FTIR) spectroscopy combined with multivariate statistical techniques. Heliyon. 2020 Apr 1;6(4):e36-62.
  17. Dyer CD. Chemical applications of Fourier-transform Raman spectroscopy (Doctoral dissertation, University of Southampton). 1995.
  18. De Oliveira LF, Colombara R, Edwards HG. Fourier transform Raman spectroscopy of honey. Applied Spectroscopy. 2002 Mar;56(3): 06-11.
  19. Mai Z, Lai B, Sun M, Shao J, Guo L. Food adulteration and traceability tests using stable carbon isotope technologies. Tropical Journal of Pharmaceutical Research. 2019;18(8): 71-84.
  20. Dranca F, Ropciuc S, Pauliuc D, Oroian M. Honey adulteration detection based on composition and differential scanning calorimetry (DSC) parameters. LWT. 2022 Oct 1;168: 113910.
  21. Angerish M. Study of Chromatography Techniques and Its Application. JETIR. 2018 Sep; 5(9): 405-411.
  22. Rohrer JS. High-performance anion-exchange chromatography with pulsed amperometric detection for carbohydrate and glycoconjugate analyses. InCarbohydrate Analysis by Modern Liquid Phase Separation Techniques. Elsevier. 2021 Jan 1 pp. 157-207.
  23. CABANero AI, Recio JL, Ruperez M. Liquid chromatography coupled to isotope ratio mass spectrometry: a new perspective on honey adulteration detection. Journal of agricultural and food chemistry. 2006 Dec 27;54(26): 19-27.
  24. Bilikova K, Simuth J. New criterion for evaluation of honey: quantification of royal jelly protein apalbumin 1 in honey by ELISA. Journal of agricultural and food chemistry. 2010 Aug 11;58(15): 76-81.
  25. Silva GS, Bernardoni V, Santana RM, Calaça P, Assis DS, Lima WG, Gardoni LC, Brito J. Official labeled and unlabeled Brazilian honey. Comparison between physicochemical, microbiological, and microscopic parameters. Revista Colombiana de Ciencias Químico-Farmacéuticas. 2021 Aug;50(2): 57-75.
  26. Ciursa P, Oroian M. Voltammetric e-tongue for honey adulteration detection. Sensors. 2021 Jul 26;21(15):50-59.
  27. Puścion-Jakubik A, Borawska MH, Socha K. Modern methods for assessing the quality of bee honey and botanical origin identification. Foods. 2020 Jul 31;9(8):10-28.